Exam Pattern & Syllabus for APPSC Biotechnology Degree Lecturers 2021

Exam Pattern & Syllabus for APPSC Biotechnology Degree Lecturers, APPSC has given the Degree College Lecturers Recruitment notification and online applications are invited online from qualified candidates to the post of Degree College Lecturers in Govt Degree Colleges in the State of Andhra Pradesh.

The proforma Application will be available on the Commission’s Website (www.psc.ap.gov.in) from 29/12/2016 to 28/01/2017 (Note: 27/01/2017 is the last date for payment of fee up- to 11:59 midnight). APPSC Degree College Lecturers Recruitment notification no.26/2016 and apply online now @ http://appscapplications17.apspsc.gov.in/

Scheme of Exam: Exam Pattern & Syllabus for APPSC Biotechnology Degree Lecturers

PapersNo. of   QuestionsDuration   (Minutes)Maximum   Marks
PART-A: Written ‘Examination (Objective Type)
Paper-1: General Studies & Mental Ability150150150
Paper-2:  Biotechnology subject150150300
PART-B: Interview (Oral Test)  50
TOTAL 500

NEGATIVE  MARKS: 
As per G.O.Ms. No.235, Finance (HRI, Plg & Policy) Dept., Dt.06/12/2016, for each wrong answer will be penalized with 1/3rd of the marks prescribed for the question.

Biotechnology Subject Syllabus: Exam Pattern & Syllabus for APPSC Biotechnology Degree Lecturers

CELL BIOLOGY: Diversity of cell size and shape. Cell theory, microscopic techniques for the study of cells. Sub-cellular fractionation and criteria of functional integrity. Cellular organelles-Plasma membrane, cell wall, Mitochondria, Chloroplast, Nucleus, and other organelles and their organization, structure, and functions. Cell motility-Cilla, flagella of eukaryotes.

Transport of nutrients, ions, and macromolecules across membranes. Liposomes, drug delivery systems, cellular energy transactions- the role of mitochondria and chloroplast. Molecular assemblies like membranes; structure and functional aspects. Ribosome’s, extracellular matrix. Cell cycle – Overview of the eukaryotic cell cycle, regulation of cell cycle by cell growth and extracellular signals. Cell cycle checkpoints. Regulators of cell cycle progression – MPF, cyclins and cyclin-dependent kinases.

Cell death and proliferation – Apoptosis: definition, differences between apoptosis and necrosis and mechanism. Cancer: Types and Classification, Development and Properties of Cancer cells. Somatic mutations in cancer cells. Biotic and abiotic stress in plants. Signal transduction: types of receptors, second messengers (calcium, phosphoinositides and Nitric oxide). Meiosis, Gametogenesis, fertilization, and development of chick embryo.

BIOMOLECULES AND ANALYTICAL TECHNIQUES: – Chemical foundations of Biology water, pH, pK, acids, bases, buffers, weak bonds, covalent bonds. Principles of thermodynamics. Classification, structure, and functions of carbohydrates, amino acids, proteins, nucleic acids, and lipids. Chromatography Methods; partition, ion exchange, and affinity methods, criteria for purity, proteins and nucleic acid sequencing methods, Hormones, vitamins, and minerals.

Analytical techniques: Principle, instrumentation, and applications of VIS/UV, IR, NMR, LASER Raman Spectroscopy MASS Spectroscopy, Fluorescence Spectroscopy, Differential calorimetry, X-ray Crystallography, Ultracentrifugation, Electron Cryomicroscopy, and Scanning Tunneling Microscopy. Methods of cell study; confocal microscopy, Flow cytometry and FACS (fluorescence-activated cell sorter) and atomic force microscopy.

Radiochemical methods; Stable and radioactive isotopes, measurement of radioactivity by Liquid scintillation counting, GM counters, and autoradiography. Specific activity and precursor-product relationship. Tracer studies and Effect of radiation on cells.

MOLECULAR BIOLOGY: – DNA Structure, replication, repair and recombination, Transcription, regulation and post-transcriptional modifications in Prokaryotes and Eukaryotes. Transcriptional and post-transcriptional gene silencing. Translation and regulation in Prokaryotes and eukaryotes, co-translation and post-translational modifications of proteins.

Protein Localization-Synthesis of secretory and membrane proteins, import into nucleus, mitochondria, chloroplast and peroxisomes, Biology of Cancer-Oncogenes and Tumour Suppressor genes, Structure, function and mechanism of action of pRB and p53 tumor suppressor proteins. Antisense oligonucleotides, molecular targets of drug action and Ribozyme Technology.

Homologous Recombination-Holliday model gene targeting, gene disruption, FLP/FRT, and Cre/Lox recombination RecA and other recombinases. Molecular Mapping of Genome, Genes, mutation and mutagenesis, site-directed mutagenesis and Human genome project, Transposons

BIOSTATISTICS: – Frequency distribution, Distribution of data binomial, poison and normal. Measures of central tendency-mean, median, mode, and standard deviation-probability distribution-regression-correlation- Analysis of variance-tests of significance- T-test, F-test, Chi-square test.

MICROBIOLOGY: – Discovery of the microbial world; Distinguishing features of prokaryotes and eukaryotes; the general role of microorganisms in transformation of organic matter and in the causation of diseases; Microbial taxonomy; Classification, Nomenclature and new approaches to microbial taxonomy; Pure culture techniques; sterilization methods; Principles of microbial nutrition and composition of culture media;

culture enrichment techniques; Growth and its mathematical expression; synchronized cultures, Culture collection, and maintenance of cultures; Purple and green bacteria Ricketsias; Chlamydia and Mycoplasma. Archea; Viruses: structure and replication of viruses; DNA viruses and RNA viruses; Viroids and Prions; Viruses and their Genetic System; Bacteriophages; RNA phages; Retroviruses, Biomass and Bioenergy.

IMMUNOLOGY:- Phylogeny of immune System; Innate and acquired immunity; Hematopoietic and differentiation, cells, and organs of the immune system; Lymphocyte trafficking; Antigencity and superantigens; Immunoglobulin types, structure, and function, Antigen-antibody interactions; Blood groups, Cell migration and Homing, Immunoglobulin and gene organization.

Major histocompatibility complex, BCR and TCR and generation of diversity; Complement system, Antigen processing, and presentation, generation of humoral and cell-mediated immune responses; Activation of B-and T- lymphocytes, Cytokines and their role in immune regulation; Cell-mediated cytotoxic, Hypersensitivity, Autoimmunity, Transplantation, Tumor Immunology,

AIDS and other Immunodeficiency; Hybridoma Technology, Psychoneuroimmunology, Single chain antibodies, theories of antibody diversity, Vaccines – Concept of immunization, routes of vaccination. Types of vaccines – Whole organism (attenuated and inactivated) and component vaccines (synthetic peptides, DNA vaccines, recombinant vaccines, subunit vaccines, conjugate vaccines. Vaccine delivery systems.

BIOPROCESS ENGINEERING:- Fermentation-types of fermentors and bioreactor design, cell concentration and stirring. Filtration, methods of cell disruption. Downstream processing, industrial applications of the bioprocess. Synchronized and continuous culturing. Industrial production of glutamic acid, citric acid, ethanol, penicillin, and riboflavin. Purification and crystallization of products.

ENZYME TECHNOLOGY:- Discovery classifications and nomenclature of enzymes. Techniques of enzymes isolation and assay, Intracellular localization of enzymes, Isoenzymes, Multienzyme complexes and multifunctional enzymes Physico-chemical characterization of enzymes, Enzymes kinetics, kinetic of enzymes of inhibition. Allosteric, Enzyme memory and mnemonic enzymes, Various techniques used for the immobilization of enzymes and their applications in Biotechnology. Purification of enzymes and their applications, Single-cell proteins. Industrial application of enzyme, applications in biosensors.

ENVIRONMENTAL BIOTECHNOLOGY:- Ecological balance, the resiliency of ecosystem and sustainable development, environmental pollution and global problems, water, air, soil pollution and their impacts on the environment and biotechnological approaches for management, wastewater treatment: aerobic and anaerobic processes, bioremediation of contaminated soils and wasteland, biotechnological treatment for industrial effluents and solid wastes.

GENETIC ENGINEERING:- Discovery, properties and application of Restriction enzymes, Cloning and expression vectors, Purification of plasmids, genomic DNA and mRNA. Genomic and cDNA Library construction and screening of recombinants by hybridization methods, Reporter assays, protein engineering- site-directed mutagenesis, adding disulfide bonds – changing asparagines to other amino acid modification of metal cofactor requirements.

Increasing of specific activity Stability to thermal and salinity conditions, Phage Display library and yeast two-hybrid systems. Gene transfer methods Tagging, Role of gene tagging analysis; Gene Therapy, Gene silencing methods, Biochips.

BIOINFORMATICS-:- Biological databases, ORF finding, EST analysis, gene identification, microsatellite repeat patterns, Blast all flavours, Mutation matrix, global Vs local alignments, Dot plots, PAM and BLOSUM matrices, Multiple sequence modeling, alignments dendrograms, phylograms, protein structure prediction methods, molecular modeling, Primer design, QSAR, Drug designing

PLANT BIOTECHNOLOGY:- Selection of explants, micro and macro Propagation techniques in plant tissue culture suspension culture, single cell. Anther, pollen and ovary culture for production of haploid plants. Cryopreservation for germplasm conservation. Plant Transformation technology, Transgene stability and gene silencing. Application of plant Transformation for productivity and performance.

Metabolic Engineering and Industrial products: Plant secondary metabolites, industrial enzymes, biodegradable plastics, therapeutic proteins, antibodies, edible vaccines. Molecular marker-assisted selection and Breeding: RFLP maps, RAPD markers, STS, microsatellites, SCAR (sequence characterized amplified regions), SSCP (single-strand conformational polymorphism), AFLP, GM Crops

ANIMAL BIOTECHNOLOGY: – Animal cell culture technology, simple and complex growth media, cell culture techniques, primary and established cell lines. Biology and characterization of the cultured cells, measuring growth parameters, maintenance of cell culture, Measurement of viability and cytotoxicity, cell separation. Scaling–up of animal cell culture. Cell synchronization. Cell cloning and micromanipulation.

Cell transformation. Stem cell cultures, embryonic stem cells and their applications. Cell culture-based vaccines. Organ and histotypic cultures. Apoptosis, measurement of cell death. Biodegradation of Toxicants, Diagnostic aids, organ perfusion  studies., GM animals.Principles and preparation of DNA and RNA probes and their applications:

Study and expression of cloned genes in prokaryotes and eukaryotic systems. Microbial production of interferon, human growth hormone, insulin in E.coli. Genetic Engineering – Social and moral implications, national and international guidelines/regulations. Biotechnology patents and safeguarding human health.

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